Aflatoxins are toxic and carcinogenic. They are metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. There are four principal types of aflatoxin: B1, B2, G1 and G2, which are named for their respective innate fluorescent properties. Aflatoxins can be found mainly in cereals, nuts, cottonseed and human blood.

The kit is an indirect competitive enzyme-labeled immunoassay. The Aflatoxin antigen is precoated on the wells. The precoated antigen competes with aflatoxin in the sample for binding the aflatoxin antibody (antibody solution). The anti-Aflatoxin antibody binds to the Aflatoxin-HRP enzyme conjugate and the resulting color change after the addition of substrate has an inverse relationship to the target analyte concentration in the sample.

Sensitivity: 0.03μg/kg

Limit of detection: 1.05μg/kg

Percent Recovery, %: 90±15%

Precision (CV %): Intra-lab assay:CV%≤10%

Qualitative or quantitative analysis of Aflatoxin B1 in samples of grains, nuts, spices, edible oil and feed.